Cytation Multi-Mode Reader

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Synergy™ Neo2 Multi-Mode Microplate Reader is designed for speed and ultra high performance, incorporating BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance. Continuously variable bandwidth quadruple monochromators, sensitive high transmission filter-based optics and up to 4 PMTs provide ultra-fast measurements with excellent results. Advanced environment controls, including available CO₂/O₂control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Barcode-labeled filter cubes help streamline workflows and limit errors, while the available BioStackNeo plate stacker is the fastest on the market - ideal for high throughput requirements. Powerful Gen5™ Data Analysis Software is included, and features designed for 21 CFR Part 11 compliance are available in Gen5 Secure.

The available BioStack Neo integrates with Synergy Neo2 for fast automated processing of up to 50 plates.

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.
HTRF® and the HTRF logo are registered trademarks of Cisbio.

Patented Hybrid Technology™ with independent filter and monochromator-based optics
Scientific quad monochromators with continuously variable bandwidth for optimal sensitivity
High performance filter system
Ultra-fast plate processing speeds with multiple PMT detectors
Live cell options: Controlled environment and direct bottom detection
Driven by best-in-class Gen5™ software for data collection and analysis
21 CFR Part 11 Compliance with available Gen5 Secure Software

Fiber-free top optics light path with dual PMT detectors

Modular and upgradable

Ultra Fast Detection Configurations include dual top PMTs for fluorescence and luminescence.

	Monochromator-based		Filter-based			Laser Alpha
	UV-Vis absorbance	top/bottom FI	FI/FP/TRF	bottom FI/FP/TRF	luminescence	top
NEO2	•		•		•
NEO2B	•		•	•	•
NEO2M	•	•	•		•
NEO2MB	•	•	•	•	•
NEO2ALPHA	•		•		•	•
NEO2ALPHAB	•		•	•	•	•
NEO2MALPHA	•	•	•		•	•
NEO2MALPHAB	•	•	•	•	•	•

Performance & Value Configurations include a single top PMT for fluorescence and luminescence.

	Monochromator-based		Filter-based			Laser Alpha
	UV-Vis absorbance	top/bottom FI	FI/FP/TRF	bottom FI/FP/TRF	luminescence	top
NEO2S	•		•		•
NEO2SB	•		•	•	•
NEO2SM	•	•	•		•
NEO2SMB	•	•	•	•	•
NEO2SALPHA	•		•		•	•
NEO2SALPHAB	•		•	•	•	•
NEO2SMALPHA	•	•	•		•	•
NEO SMALPHAB	•	•	•	•	•	•

General
Detection modes	UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	6- to 1536-well plates
Other labware supported	Petri and cell culture dishes Take3 Micro-Volume Plates
Temperature control	4-Zone incubation to 65 °C with Condensation Control™ +0.2 °C at 37 °C
Shaking	Linear, orbital, double orbital
Software	Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation	BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible
CO₂ and O₂ control (option)	Range: 0 - 20% (CO₂); 1 - 19% (O₂) Control Resolution: + 0.1% (CO₂ and O₂) Stability: +0.2% at 5% CO₂; +0.2%at 1% O₂ Models for CO₂ /O₂ or CO₂ only are available
Barcode reader	1D and 2D camera-based scanner
Absorbance
Light source	Xenon flash
Detector	photodiode
Wavelength selection	monochromator
Wavelength range	230 - 999 nm, 1 nm increments
Monochromator bandwidth	2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 2.0 OD <3% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds
Fluorescence Intensity
Light source	Xenon flash
Detector	Dual top PMTs Single top PMT (option) Low noise PMT (bottom filter system) Red shifted PMT (top/bottom monochromator system)
Wavelength selection	Quad monochromators (top/bottom) Filters (top/bottom)
Wavelength range	Monochromators: 250 - 850 nm Filters (dual PMT): 200 - 850 nm
Monochromator bandwidth	Variable, from 3 to 50 nm in 1 nm increments
Dynamic range	7 decades
Sensitivity	Filters: Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) - top Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) - top Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) - bottom Quad monochromator: Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) - top Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) - bottom
Reading speed (kinetic)	96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds
Luminescence
Wavelength range	300 - 700 nm
Dynamic range	> 6 decades
Sensitivity	5 amol ATP typical (384-well low volume plate)
Fluorescence Polarization
Light source	Xenon flash
Detector	Dual PMT or single PMT (option)
Wavelength selection	Filters
Wavelength range	280 - 850 nm
Sensitivity	1 mP standard deviation at 1 nM fluorescein (384-well low volume plate) 1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Time-Resolved Fluorescence
Light source	Xenon flash
Detector	Dual PMT or single PMT (option)
Wavelength selection	Quad monochromators (top/bottom) Filters (top/bottom)
Wavelength range	Monos: 250 - 850 nm Filters (dual PMT): 200 - 850 nm
Sensitivity	Europium 40 fM (384-well low volume plate) Europium 70 fM (1536-well plate)
Alpha Detection
Light source	100 mW 680 nm laser
Detector	PMT
Wavelength selection	Filters (top)
Sensitivity	100 amol LCK peptide (384-well low volume plate)
Read speed	96 well: 30 seconds 384 well: 1 minute 50 seconds 1536 well: 7 minutes 20 seconds
Reagent Dispensers
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri dishes
Dead volume	1.1 ml, 100 µL with back flush
Dispense volume	5 - 1000 µL in 1 µL increments
Dispense accuracy	+ 1 µL or 2%
Dispense precision	< 2% at 50-200 µL
Physical Characteristics
Power	250 Watts max.
Dimensions	15.4" W x 20.7" D x 16.1" H (39 x 52.5 x 41 cm)
Weight	78 lbs (35 kg)
Regulatory
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

BioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Note: Some microplate readers come with a specific edition of Gen5, please check the microplate reader specifications.

Optional

BioSpa 8 Automated Incubator

BioSpa 8 is an automated incubator linking BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO₂/O₂ and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Optional
BioSpa 8 Integration Kits
1410008	Alignment kit for Synergy Neo2 and Cytation 3/5
BioStack Integration Kits
7310055	BioStack integration kit for Synergy Neo2
Filter Cubes
1035001	Alpha 96/384-well plates 570/100 nm emission
1035002	Alpha top cube for dual PMT system
1035003	Top single PMT fluorescence/ Luminescence
1035004	All Dual FP assays
1035005	Alpha 1536-well plates 570/100 nm emission
1035006	AlphaPlex lower cube #6
1035011	HTRF-Europium excitation 330 nm
1035012	HTRF Terbium / LanthaScreen Terbium excitation 340 nm
1035013	CFP-YFP FRET excitation 420 nm
1035014	FITC-TRITC, EGFP-Cy3, EGFP-YFP FRET excitation 485 nm
1035015	Cy3-Cy5 FRET excitation 540 nm
1035016	GeneBLAzer, Z-Lyte excitation 400 nm
1035017	LANCE excitation 360 nm
1035041	HTRF, LANCE red emission 620/665 nm
1035042	LanthaScreen emission 495/520 nm
1035043	CFP-YFP FRET emission 485/540
1035044	FITC-TRITC FRET emission 528/575 nm
1035045	Cy3-Cy5 FRET emission 590/680
1035046	EGFP-Cy3 FRET emission 516/590
1035047	EGFP-YFP FRET emission 516/540
1035048	GeneBLAzer, Z-Lyte emission 460/528
1035049	HTRF green emission 520/620
1035061	Dual FP Green 485/528
1035062	Dual FP red1 530/590
1035063	Dual FP red2 590/635
1035064	Dual FP far red 620/680
1035066	Dual FP UV 360/460
1035067	Dual Filter Cube UV 340/530
1035071	BRET1 Dual PMT
1035072	BRET2 Dual PMT
1035073	AlphaPlex upper cube #73
1035101	GFP excitation / emission – luminescence
1035102	GeneBLAzer
1035103	FITC-TRITC FRET
1035104	EGFP-Cy3 FRET
1035105	EGFP-YFP FRET
1035106	Cy3-Cy5 FRET
1035107	Blue (360/460) – Green (485/528) fluorescence
1035108	Green FP 485/528
1035109	BRET2 410/515
1035110	Far red FP 620/680
1035111	Green (485/528) – Red (530/590) fluorescence
1035112	Delfia Europium 360/620
1035113	Omnia (360/485) / Luminescence
1035114	Luminescence standard / 1536
1035115	Red (530/590) – Far Red (620/680)
1035116	Fura 2 (Ex 340, 380; Em 508)
1035117	BRET 1 cube, 460/40 and 540/25 nm
1035118	Deep UV FP 284/340 for use with Synergy Neo
1035119	Single PMT HTRF Red
1035120	Single PMT FP 530/590 FP
1035121	Single PMT Lum 485/Lum 600
1035255	Custom cube #255
Miscellaneous
1030008	Barcode Reader Option
1210012	CO₂ - only gas controller for Synergy Neo2
1210013	CO₂ and O₂ gas controller for Synergy Neo2
Product Qualification Packages
1350526	Synergy Neo2 Product Qualification Package - IQ/OQ/PQ
Reagent Injector Assembly
8040030	Dual reagent injector module
Reagent Injector Options
1320514	Angled tip injector head
Software Options
GEN5SECURE	Gen5 Secure
GEN5IVD	Gen5 IVD
GENRC	Gen5 Reader Control (RC)
Take3 Micro-Volume Plate
TAKE3	Take3 Micro-Volume Plate
Take3TRIO	Take3 Trio Micro-Volume Plate
Test Plates & Reagents
7260522E	Absorbance Test Plate
7260551	340 nm Absorbance Test Plate
8030015	Luminescence Test Plate