| General | 
  | Detection modes | 
  UV-Vis absorbance 
Fluorescence intensity 
Luminescence 
Fluorescence polarization 
Time-resolved fluorescence 
Alpha | 
  | Read methods | 
  Endpoint, kinetic, spectral scanning, well area scanning | 
  | Microplate types | 
  Monochromator: 6- to 384-well plates 
Filters: 6- to 1536-well plates 
Imaging: 6- to 1536-well plates | 
  | Other labware supported | 
  Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) 
Take3 Micro-Volume Plates | 
  | Temperature control | 
  4-Zone incubation to
65 °C with Condensation
Control; +0.2 °C at 37 °C | 
  | Shaking | 
  Linear, orbital, double orbital | 
  | Software | 
  Gen5™ Microplate Reader and Imager Software 
Gen5 Secure for 21 CFR Part 11 compliance (option) | 
  | Automation | 
  BioStack and 3rd party automation compatible 
BioSpa 8 Automated Incubator compatible | 
  | CO2 and O2 control (option) | 
  Range: 0 - 20% (CO2); 1 - 19% (O2) 
Control Resolution: +0.1% (CO2 and O2)  Stability: +0.2% at 5% CO2; +0.2%
at 1% O2 
Models for both CO2 and O2 or CO2 only are available | 
| Imaging System | 
  | Imaging mode | 
  Fluorescence 
Brightfield 
Color Brightfield 
Phase Contrast | 
  | Imaging method | 
  Single color, multi-color, montage, time lapse, z-stacking | 
  | Image processing | 
  Z-projection, digital phase contrast, stitching | 
  | Camera | 
  16-bit gray scale, Sony CCD, 1.25 megapixel.                                                 0.3 µm/pixel at 20x | 
  | Objective capacity | 
  6 user-replaceable
objectives | 
  | Objectives available | 
  1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x | 
  | Phase objectives available | 
  4x, 10x, 20x, 40x | 
  | Image filter cube capacity | 
  4 user-replaceable fluorescence cubes plus brightfield channel | 
  | Imaging filter cubes available | 
  DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 | 
  | Imaging LED cubes available | 
  365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm | 
  | Automated functions | 
  User-trained autofocus, autofocus, autoexposure,
auto-LED intensity | 
  | Autofocus method | 
  Image-based autofocus 
User-trained autofocus 
Laser autofocus (option) | 
  | Positional controls | 
  Software control 
Joystick control (option) | 
  | Image collection rate | 
  Image-based autofocus: 
96 wells, 1 color (DAPI), 4x, 6 minutes 
96 wells, 3 colors, 4x, 12 minutes
  
Laser autofocus: 
96 wells, 1 color (DAPI), 4x, <3 minutes 
96 wells, 3 colors, 4x, <7 minutes, 30 seconds | 
  | Image Analysis Software option | 
  Gen5 Image+: Advanced image analysis 
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features | 
| Fluorescence Intensity | 
  | Light source | 
  Xenon flash | 
  | Detector | 
  PMT for monochromator system 
PMT for filter system | 
  | Wavelength selection | 
  Quad monochromators (top/bottom) 
Filters (top) | 
  | Wavelength range | 
  Monochromators: 250 - 700 nm (850 nm option) 
Filters: 200 - 700 nm (850 nm option) | 
  | Monochromator bandwidth | 
  Variable, from 9 nm to 50 nm in 1 nm increments | 
  | Dynamic range | 
  7 decades | 
  | Sensitivity | 
  Filters: 
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)   
Quad Monochromator: 
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top 
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom | 
  | Detection system | 
  Two PMT detectors: one for monochromator system, one for filter system | 
  | Reading speed (kinetic) | 
  96 wells: 11 seconds 
384 wells: 22 seconds | 
| Luminescence | 
  | Wavelength range | 
  300 - 700 nm | 
  | Dynamic range | 
  >6 decades | 
  | Sensitivity | 
  Monos: 20 amol ATP (flash) 
Filters: 10 amol ATP (flash), 100 amol (glow) | 
| Fluorescence Polarization | 
  | Light source | 
  Xenon flash | 
  | Detector | 
  PMT | 
  | Wavelength selection | 
  Filters | 
  | Wavelength range | 
  280 - 700 nm (850 nm option) | 
  | Sensitivity | 
  1.2 mP standard deviation at 1 nM fluorescein | 
| Time-Resolved Fluorescence | 
  | Light source | 
  Xenon flash | 
  | Detector | 
  PMT | 
  | Wavelength selection | 
  Quad monochromators (secondary mode) 
Filters (top) | 
  | Wavelength range | 
  Filters: 200 - 700 nm (850 nm option) | 
  | Sensitivity | 
  Filters:  Europium 40 fM (4 amol/well, 384-well plate) 
Monos: Europium 1200 fM (120 amol/well, 384-well plate) | 
| Absorbance | 
  | Light source | 
  Xenon flash | 
  | Detector | 
  photodiode | 
  | Wavelength selection | 
  monochromator | 
  | Wavelength range | 
  230 - 999 nm, 1 nm increment | 
  | Monochromator bandwidth | 
  4 nm (230 - 285 nm), 8 nm (>285 nm) | 
  | Dynamic range | 
  0 - 4.0 OD | 
  | Resolution | 
  0.0001 OD | 
  | Pathlength correction | 
  yes | 
  | Monochromator wavelength accuracy | 
  +2 nm | 
  | Monochromator wavelength repeatability | 
  +0.2 nm | 
  | OD accuracy | 
  <1% at 2.0 OD 
<3% at 3.0 OD | 
  | OD linearity | 
  <1% from 0 to 3.0 OD | 
  | OD repeatability | 
  <0.5% at 2.0 OD | 
  | Stray light | 
  0.03% at 230 nm | 
  | Reading speed (kinetic) | 
  96 wells: 11 seconds 
384 wells: 22 seconds | 
| Alpha Detection | 
  | Light source | 
  100 mW 680 nm laser | 
  | Detector | 
  PMT | 
  | Wavelength selection | 
  Filters (top) | 
  | Sensitivity | 
  100 amol LCK peptide (384-well low volume plate) | 
| Reagent Injectors (option) | 
  | Supported detection modes | 
  All modes | 
  | Number | 
  2 syringe pumps | 
  | Supported labware | 
  6- to 384-well plates, Petri dishes | 
  | Dead volume | 
  1.1 mL with back flush | 
  | Dispense volume | 
  5 - 1000 µL in 1 µL increments | 
  | Plate geometry | 
  6- to 384-well microplates, Petri dishes | 
  | Dispense accuracy | 
  +1 µL or 2% | 
  | Dispense precision | 
  <2% at 50 - 200 µL | 
| Physical Characteristics | 
  | Power | 
  250 Watts max. | 
  | Dimensions | 
  20" D x 16.5" W x 17.5" H 
(50.8 cm x 41.91 cm x 44.5 cm) | 
  | Weight | 
  80 lbs (36.3 Kg) | 
| Regulatory | 
  | Regulatory | 
  CE and TUV marked. RoHS Compliant. Models for In Vitro
Diagnostic use are available. |